Journal: Microbiology

Article Title: ChpC controls twitching motility-mediated expansion of Pseudomonas aeruginosa biofilms in response to serum albumin, mucin and oligopeptides

doi: 10.1099/mic.0.000911

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: [ 60 ] pGEMTeasy AmpR; E. coli cloning vector Promega pGemCLF AmpR; A 1149 bp PCR amplicon from the 5′ end of chpC (CLF) and inserted into pGEMTeasy This study pGemCRF AmpR; A 1238 bp fragment PCR amplicon from the 3′ end of chpC (CRF) and inserted into pGEMTeasy This study pCRFV KanR; Bam HI- Kpn I fragment from pGEMCRF inserted into pOK12 This study pOKCDEL Kan, TcR; The concatamerized fragment including the Bgl II- Kpn I fragment from pGEMCLF and the 1638 bp KpnI fragment from pLM1 with a frt flanked TcR cassette, inserted into the Kpn I and Bam HI sites of pCRFV fragment; This study pRIC380 AmpR; P. aeruginosa suicide vector [ 58 ] pCDEL AmpR, TcR; A 4008 bp Spe I fragment from pOKCDEL containing the chpC deletion construct inserted into the Spe I site in pRIC380 against sacB This study Open in a separate window Strains and plasmids used in this study The PAK genome (NCBI number accession number {"type":"entrez-nucleotide","attrs":{"text":"LR657304","term_id":"1708254220","term_text":"LR657304"}} LR657304 [ 48 ];) was used to search for methyl-accepting chemotaxis protein (MCP) and solute-binding protein (SBP) orthologs.

Techniques: Plasmid Preparation, Amplification, Clone Assay, Construct

Journal:

Article Title: Transcription of All amoC Copies Is Associated with Recovery of Nitrosomonas europaea from Ammonia Starvation ▿

doi: 10.1128/JB.01861-06

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: pCRII , Dual promoter TA PCR cloning vector (Kan r Amp r lacZ α f1 ori ColE1 ori ) , Invitrogen.

Techniques: Plasmid Preparation, Acetylene Reduction Assay, PCR Cloning, Clone Assay

Journal: Journal of biotechnology

Article Title: Isolation, cloning and characterisation of the abiI gene from Lactococcus lactis subsp. lactis M138 encoding abortive phage infection

doi:

Figure Lengend Snippet: Bacterial strains, plasmids and phages

Article Snippet: Lactose utilisation, proteinase activity and nisin resistance were determined by growth on solid media for 48 h. When appropriate, antibiotics were added as follows: for E. coli, 50 μ g ampicillin ml −1 ; for L. lactis, 5 μ g erythromycin ml −1 , 5 μ g chloramphenicol ml −1 , 300 IU nisin ml −1 , 50 μ g fusidic acid ml −1 and 500 μ g streptomycin ml −1 . table ft1 table-wrap mode="anchored" t5 caption a7 Strain, plasmid or phage Relevant characteristics Source or Reference Bacteria L. lactis subsp. lactis M138 Industrial strain, Lac + Prt + Nis r Mauri Laboratories, Moorebank, Australia LM0230 Lac − Prt − , plasmid-free derivative of c2 Efstathiou and McKay, 1977 LM0230Sm r Sm r derivative of LM0230 Duan et al., 1996 LM0230Fus r Fus r derivative of LM0230 Duan et al., 1996 E. coli NM522 Transformation host Gough and Murray, 1983 Plasmids pGB301 9.8 kb streptococcal vector, Em r , Cm r Behnke and Gilmore, 1981 pGEM7Zf(+) 3 kb E.coli vector encoding Amp r and lacZ screening Promega Corporation, Madison, WI pMU1327 7.0 kb streptococcal vector, Em r Achen et al., 1986 pND852 Native plasmid of M138, φ r Nis r , 56 kb This study pND817 12.3 kb, 2.6 kb Hpa II fragment from pND852 cloned into the Hpa II site of pGB301 This study pND821 5.6 kb, 2.6 kb Hpa II fragment from pND817 cloned into the Cla I site of pGEM-7Zf(+) This study pND835 1225 bp Alu I fragment encoding abiI cloned into pMU1327 at the Sma I site This study pND839 Frame-shift mutation at the unique Eco RI site of pND817 This study Phages φ 712 Small isometric-headed phage propagated on LM0230 M.J. Gasson, AFRC Institute of Food Research, Norwich, UK φ c2 Prolate-headed phage propagated on LM0230 L.L.

Techniques: Plasmid Preparation, Bacteria, Transformation Assay, Clone Assay, Mutagenesis

Journal: International Journal of Molecular Medicine

Article Title: Clinical significance of interleukin-6 and inducible nitric oxide synthase in ketamine-induced cystitis

doi: 10.3892/ijmm.2017.3264

Figure Lengend Snippet: Immunodetection of collagens from human foreskin-derived stromal cells following cyclic ketamine treatment. (A) Detection of COL1. (B) Semi-quantification of immunoblotting. Expression was semi-quantified to GAPDH and log 2 values were calculated. * P<0.05 and ** P<0.01. (C) Fluorescent detection of COL2 from cells without ketamine treatment. (D) Fluorescent detection of COL2 from cells following ketamine treatment. Isolated cells were harvested and seeded on fibronectin-coated culture plates and were then treated with the myogenic differentiation agent, containing TGF-β1 (2.5 ng/ml) and PDGF-BB (5 ng/ml). After a 14-day incubation for differentiation, cells were treated with (+) or without (-) ketamine (10 µ g/ml) for various cycles (1, 3 and 5 cycles). Each cycle composed 1 day ketamine treatment followed by 1 day culturing in medium alone. COL1, type I collagen; COL2, type II collagen; DAPI, 4',6-diamidino-2-phenylindole; PDGF-BB, platelet-derived growth factor-BB; TGF-β1, transforming growth factor-β1.

Article Snippet: The bands were visualized using a VECTASTAIN ABC-AmP Chemiluminescence Detection kit (AK-6601; Vector Laboratories, Inc.) for COL1 (10 min at 25°C) and Western Lightning Plus-ECL (#NEL103001EA; PerkinElmer, Inc., Waltham, MA, USA) for GAPDH, according to the manufacturers' protocols.

Techniques: Immunodetection, Derivative Assay, Western Blot, Expressing, Isolation, Incubation

Journal: International Journal of Molecular Medicine

Article Title: Clinical significance of interleukin-6 and inducible nitric oxide synthase in ketamine-induced cystitis

doi: 10.3892/ijmm.2017.3264

Figure Lengend Snippet: Molecular pathogenesis of ketamine-induced cystitis. Red lines or arrows indicate effects of CTX or IFS; blue lines or arrows indicate effects of ketamine. COL1, type I collagen; COL2, type II collagen; CTX, cyclophosphamide; IFS, ifosfamide; IL-6, interleukin-6; iNOS, nitric oxide synthase 2.

Article Snippet: The bands were visualized using a VECTASTAIN ABC-AmP Chemiluminescence Detection kit (AK-6601; Vector Laboratories, Inc.) for COL1 (10 min at 25°C) and Western Lightning Plus-ECL (#NEL103001EA; PerkinElmer, Inc., Waltham, MA, USA) for GAPDH, according to the manufacturers' protocols.

Techniques:

Journal:

Article Title: The Flagellar Filament of Rhodobacter sphaeroides : pH-Induced Polymorphic Transitions and Analysis of the fliC Gene

doi:

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: pUC19 , E. coli -specific cloning vector lacZ Amp r , New England Biolabs.

Techniques: Plasmid Preparation, Mutagenesis, Cloning, Clone Assay, Control

Journal:

Article Title: The Flagellar Filament of Rhodobacter sphaeroides : pH-Induced Polymorphic Transitions and Analysis of the fliC Gene

doi:

Figure Lengend Snippet: Prettybox multiple sequence alignment of R. sphaeroides FliC (flic_rsph) with FliCs from enteric and soil bacteria. For reasons of space, much of the variable central region is not shown. The regions predicted to form coiled coils are shown using serovar Typhimurium coordinates (heavy bars) or R. sphaeroides coordinates (dotted line). Accession numbers are as follows: S. meliloti FlaA (flaa_rhime), P13118; S. meliloti FlaB (flab_rhime), P13119; P. aeruginosa FlaA (flaa_pseae), P21184; B. subtilis FliC (flic_bacsu), P02868; serovar Typhimurium FliC (flic_salty), P06179; E. coli FliC (flic_ecoli), P04949.

Article Snippet: pUC19 , E. coli -specific cloning vector lacZ Amp r , New England Biolabs.

Techniques: Sequencing, Bacteria

Journal:

Article Title: The Flagellar Filament of Rhodobacter sphaeroides : pH-Induced Polymorphic Transitions and Analysis of the fliC Gene

doi:

Figure Lengend Snippet: Consensus ς28 recognition sequences compared to the putative promoter sequence of R. sphaeroides fliC. The B. subtilis ς28 is also known as ςD, and that of E. coli is known as ςF (4, 12, 21).

Article Snippet: pUC19 , E. coli -specific cloning vector lacZ Amp r , New England Biolabs.

Techniques: Sequencing